| Filename | Total | Flagged As Poor Quality | Sequence Length | %GC |
|---|---|---|---|---|
| Q1Aligned.sortedByCoord.out.bam | 80,528,674 | 0 | 8-150 | 43 |
| Q2Aligned.sortedByCoord.out.bam | 92,097,647 | 0 | 8-150 | 43 |
| Q4Aligned.sortedByCoord.out.bam | 103,912,111 | 0 | 8-150 | 43 |
| Q5Aligned.sortedByCoord.out.bam | 86,862,161 | 0 | 3-150 | 44 |
| W1Aligned.sortedByCoord.out.bam | 92,264,849 | 0 | 8-150 | 43 |
| W2Aligned.sortedByCoord.out.bam | 87,720,625 | 0 | 8-150 | 44 |
| W3Aligned.sortedByCoord.out.bam | 90,411,804 | 0 | 8-150 | 43 |
| W4Aligned.sortedByCoord.out.bam | 105,852,840 | 0 | 7-150 | 43 |
| W5Aligned.sortedByCoord.out.bam | 82,170,056 | 0 | 8-150 | 43 |
Library Sizes ranged between 80,528,674 and 105,852,840 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Hsapiens Genome
GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Genome
Total overrepresented sequences for each library
## Overrepresented_sequences missing from Q1Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from Q2Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from Q4Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from Q5Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from W1Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from W2Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from W3Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from W4Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from W5Aligned.sortedByCoord.out.bam
## No overrepresented sequences were detected by FastQC
Universal Adapter Content
## Kmer_Content missing from Q1Aligned.sortedByCoord.out.bam
## Kmer_Content missing from Q2Aligned.sortedByCoord.out.bam
## Kmer_Content missing from Q4Aligned.sortedByCoord.out.bam
## Kmer_Content missing from Q5Aligned.sortedByCoord.out.bam
## Kmer_Content missing from W1Aligned.sortedByCoord.out.bam
## Kmer_Content missing from W2Aligned.sortedByCoord.out.bam
## Kmer_Content missing from W3Aligned.sortedByCoord.out.bam
## Kmer_Content missing from W4Aligned.sortedByCoord.out.bam
## Kmer_Content missing from W5Aligned.sortedByCoord.out.bam
## No Kmers were detected by FastQC